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lentiviral backbone vector plko 1  (Addgene inc)


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    Structured Review

    Addgene inc lentiviral backbone vector plko 1
    Lentiviral Backbone Vector Plko 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+lentiviral+vector+backbone/pLKO%2E1+puro+(Plasmid+%238453)/pmc12959293-96-1-5
    Average 96 stars, based on 1561 article reviews
    lentiviral backbone vector plko 1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Control:

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. .. Replication-deficient lentiviral vector particles (LVPs) were produced by transient cotransfection of HEK 293T/17 cells (ATCC CRL-11268) with pLKO.1–short hairpin constructs along with the psPAX2 and pMD2.G helper plasmids (Addgene 12260, 12259) using TurboFect transfection reagent (Thermo Fisher Scientific).

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. ..

    Cloning:

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. .. Replication-deficient lentiviral vector particles (LVPs) were produced by transient cotransfection of HEK 293T/17 cells (ATCC CRL-11268) with pLKO.1–short hairpin constructs along with the psPAX2 and pMD2.G helper plasmids (Addgene 12260, 12259) using TurboFect transfection reagent (Thermo Fisher Scientific).

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. ..

    Plasmid Preparation:

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. .. Replication-deficient lentiviral vector particles (LVPs) were produced by transient cotransfection of HEK 293T/17 cells (ATCC CRL-11268) with pLKO.1–short hairpin constructs along with the psPAX2 and pMD2.G helper plasmids (Addgene 12260, 12259) using TurboFect transfection reagent (Thermo Fisher Scientific).

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. ..

    Sequencing:

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. .. Replication-deficient lentiviral vector particles (LVPs) were produced by transient cotransfection of HEK 293T/17 cells (ATCC CRL-11268) with pLKO.1–short hairpin constructs along with the psPAX2 and pMD2.G helper plasmids (Addgene 12260, 12259) using TurboFect transfection reagent (Thermo Fisher Scientific).

    Article Title: Mutations in tropomyosin 4 underlie a rare form of human macrothrombocytopenia
    Article Snippet: .. Short hairpin or control nontarget sequences were annealed before cloning into the pLKO.1 lentiviral vector backbone (Addgene 10878) downstream of the human U6 promoter and checked for sequence integrity. ..



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